| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Geelen, Lara (13)
|
MRRYE |
26.58 |
26.93 |
0.35 |
| 2 |
Luecke, Kendall R (13)
|
CTPSDY |
27.92 |
27.96 |
0.04 |
| 3 |
Shopovick, Sophia F (13)
|
MRYMID |
28.18 |
28.20 |
0.02 |
| 4 |
Frankowski, Paula (13)
|
CTPSDY |
29.81 |
28.80 |
-1.01 |
| 5 |
Goldaber, Hannah I (14)
|
MRLIAC |
29.17 |
29.05 |
-0.12 |
| 6 |
Olvany, Kelsey C (13)
|
CTPSDY |
27.81 |
29.31 |
1.50 |
| 7 |
Zhang, Michelle M (13)
|
MRLIAC |
30.91 |
29.50 |
-1.41 |
| 8 |
Leroy, Helena (13)
|
MRGAEL |
29.41 |
29.61 |
0.20 |
| 9 |
Chia, Molly (14)
|
MRYMID |
29.72 |
29.71 |
-0.01 |
| 10 |
Laurenceau, Alyssa M (14)
|
MRYMID |
29.23 |
30.31 |
1.08 |
| 11 |
Smith, Caroline K (13)
|
MRYMID |
29.78 |
30.46 |
0.68 |
| 12 |
McCauley, Molly A (13)
|
MRGAEL |
32.04 |
31.08 |
-0.96 |
| 13 |
Goldaber, Sarah K (13)
|
MRLIAC |
31.23 |
32.37 |
1.14 |
| 14 |
Bergamini, Sara E (14)
|
MRGAEL |
31.51 |
32.79 |
1.28 |
| 15 |
Somma, Angelina (13)
|
MRLIAC |
31.93 |
32.90 |
0.97 |
| 16 |
Kieran-Mendez, Emily M (13)
|
CTPSDY |
37.85 |
37.68 |
-0.17 |
| 17 |
Arellano, Emilia L (14)
|
MRYMID |
|
41.20 |
- |
|
Skenderi, Gia G (13)
|
MRLIAC |
44.28 |
NS |
- |
|
Nunez, Cassandra (14)
|
MRGAEL |
|
NS |
- |